Measurement

Part:BBa_K3380600:Design

Designed by: Alexandru Popov   Group: iGEM20_Edinburgh   (2020-10-10)


TXO ArsR regulated construct under T7 promoter (BBa_K3380103) expressing iSpinach


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 25


Design Notes

All the sequences for the individual parts were acquired. The individual part were designed with 4bp scars to allow swapping of the parts to test different constructs. The individual parts were phosphorylated, annealed and ligated together using the T4 ligase.

Source

Cuprividus Metallidurans CH34 for the cell extract. The parts were purchased as de novo synthesized sequences.

References